]), in 70% v/v EtOH at varying temperatures from 20 to 40 °C in steps of 5 °C was measured by incubating lipids with 70% v/v EtOH in separate containers on a thermoshaker using 3 h of shaking and 1 h of rest pr. temperature level. Samples of the lipid mixtures were drawn at each temperature and dried .
To measure absolute mass of bone mineral, lipid, and lean tissue for each segment of the specimen, slice-wise proportions of each substance was multiplied by the modeled density of the substance at the given depth . Lean muscle density [ ] was adjusted to pressure and temperature by the same factor as sea water. To measure absolute buoyancy for each segment, the volume displaced by the segment was multiplied by density of Comoran sea water at the given depth.